High-Performance Liquid Chromatography (HPLC)
High-performance liquid chromatography (HPLC) is a method that separates and detects components in a liquid by utilizing the differences in interactions between the stationary phase and the mobile phase.
- Compounds that are relatively low molecular weight and highly polar, such as organic acids, can also be analyzed. - Low polarity compounds, such as lipids, can also be analyzed. - By using the post-column method, it is possible to eliminate the effects of interfering components that could not be completely separated. - Quantification of polymers and measurement of molecular weight distribution are possible.
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